'Cayuga White' x 'Aurore' Primers

Primers Used for RAPD Analysis of
'Cayuga White' x 'Aurore'

Primer Nucleotide Sequence

B302 CGG CCC ACG T

B312 ACG GCG TCA C

B315 GGT CTC CTA G

B318 CGG AGA GCG A

B324 ACA GGG AAC G

B326 CGG ATC TCT A

B336 GCC ACG GAG A

B341 CTG GGG CCG T

B345 GCG TGA CCC G

B349 GGA GCC CCC T

B350 TGA CGC GCT C

B352 CAC AAC GGG T

B355 GTA TGG GGC T

B356 GCG GCC CTC T

B358 GGT CAG GCC C

B363 ATG ACG TTG A

B372 CCC ACT GAC G

B374 GGT CAA CCC T

B375 CCG GAC ACG A

B379 GGG CTA GGG T

B388 CGG TCG CGT C

B389 CGC CCG CAG T

B391 GCG AAC CTC G

B396 GAA TGC GAG G

GTO3 CTC GGT ACA C

GTO4 GTG GTT GCG A

GTO5 CTG GAC TTA C

GTO6 ATG TGG TGG T

K04 GAT GGT ACC G

K05 CGC AGG ATG G

K08 CCC ATG GGT G

OP01 CAG GCC CTT C

OP07 GAA ACG GGT G

OP08 GTG ACG TAG G

OP09 GGG TAA CGC C

OP10 GTG ATC GCA G

OP13 CAG CAC CCA C

OP18 AGG TGA CCG T

OP20 GTT GCG ATC C

P01 CCT GTA GTG G

P04 GTT AGG TCG T

P06 TCG CCC CAT T

P07 CGT GGT TCC C

P08 GTC CCG TTA C

P09 ACG CCC TAG T

P11 CTG TGC TGT G

P12 TGG TGG ATG TA

P13 GGT GAT GTC C

P14 TGC CTT CCA T

P15 GGG TTG CCG T

P21 CCC TGT CTC T

P27 ACC TCG AGC A

P28 TCG TAG CCA A

P33 GTA AAA CGA CGG CCA GT

P35 TGC GCA ACG TTG TTG

P36 ATA CCA AAC GAC GAG

P98 GAA CGA CGC A

P105 CAG TCG CGT G

P117 GGG ATC GTG T

P136 AGC CTC GAT T

P137 ATC TGC GAC A

P145 TAG CGG CTA C

P146 TGA CAT GCG A

P153 AAG CGA TGT T

P161 CGG ATG CCT T

P163 ACG CCT ACG T

P166 GTG ACG GAC T

P210 AAA TGC GGC A

P217 CGC TCA TTC G

P220 ACA GTG CTG G

P229 CAG CAT CCC A

P231 ATG ACG CTC C

P232 CCG CTT GTT G

P242 ATG ATA CGC C

P245 CTA GGT CTC C

P252 TCA GGC TTC C

P255 ACG TTT TCC C

P265 ACG ACA GGC T

P300 ATT GCA GCC A

P312 GTG CGC CTA A

P313 AAA GCC GTC C

P314 GGT GCT CTG A

P319 ACT ACA CGA C

P321 GTT TCG CAC C

P322 CTC CAG ACA G

P323 GTG CAC CCT T

P324 CAC GCC AAA G

P326 TAC GTG CTG G

P348 CGA AAA GCG G

P353 ATA CGG GCA A

P363 AGG TGG TTC C

P371 GCC GCT TAG T

P372 AAC ACG GTC A

P379 AAC TCC GGG A

P389 GTC AGG GTG A

P391 AGA TGG GAG G

P394 CGA CTC CAA C

P395 CTG GTT CTG T

P402 GCG TTG TCC A

P414 AAG TGA GGC A

P437 CGG ATC GAC A

P441 ACC GAG TCG A

P482 AGT CGG GCA A

S05 CCG GCT CTT G

S11 CAG TGG AAT G

S12 GCG ACG CCT A

S16 CGT TGG ATG C

S19 TAC GGC TGG C

S21 CCC TAC CGA C

S23 CCA CGC TAT A

S29 CCA GAC AAG C

S32 CCC ACG GAT C

S34 GAT AGC CGA C

S35 AGT CGC TCA T

S39 TCG GCC TGC T

S41 GCC GAA AAG C

S42 CCC AGA ACA C

S44 ATT CGG CCG C

S45 CAC GTC GGA G

S60 CCG CCT CCT T

S62 GAT CCG CGT G

S64 AAC TGG CGA C

S66 GCT CAC CCT A

S69 CAT CGA ACC G

Primers Used in Combination

Combination Code Primer Combination

C03 B305 + B389

C04 P252 + B389

C06 P265 + B389

C07 B388 + S60

C08 B388 + B389

C09 OP07 + OP09

C10 OP03 + OP12

C13 P312 + P348

C14 B302 + K05

C15 B302 + K08

C16 B389 + K05

C19 P217 + K08

The primers were synthesized at the New York State Center for Advanced Technology in Biotechnology, Cornell University, Ithaca, NY; University of British Columbia, British Columbia, Canada; National Bioscience (Plymouth, MN); Operon Tech (Alameda, CA); and Genosys Biotech, Inc. (The Woodlands, TX).

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Primer	Nucleotide Sequence
B302	CGG CCC ACG T
B312	ACG GCG TCA C
B315	GGT CTC CTA G
B318	CGG AGA GCG A
B324	ACA GGG AAC G
B326	CGG ATC TCT A
B336	GCC ACG GAG A
B341	CTG GGG CCG T
B345	GCG TGA CCC G
B349	GGA GCC CCC T
B350	TGA CGC GCT C
B352	CAC AAC GGG T
B355	GTA TGG GGC T
B356	GCG GCC CTC T
B358	GGT CAG GCC C
B363	ATG ACG TTG A
B372	CCC ACT GAC G
B374	GGT CAA CCC T
B375	CCG GAC ACG A
B379	GGG CTA GGG T
B388	CGG TCG CGT C
B389	CGC CCG CAG T
B391	GCG AAC CTC G
B396	GAA TGC GAG G
GTO3	CTC GGT ACA C
GTO4	GTG GTT GCG A
GTO5	CTG GAC TTA C
GTO6	ATG TGG TGG T
K04	GAT GGT ACC G
K05	CGC AGG ATG G
K08	CCC ATG GGT G
OP01	CAG GCC CTT C
OP07	GAA ACG GGT G
OP08	GTG ACG TAG G
OP09	GGG TAA CGC C
OP10	GTG ATC GCA G
OP13	CAG CAC CCA C
OP18	AGG TGA CCG T
OP20	GTT GCG ATC C
P01	CCT GTA GTG G
P04	GTT AGG TCG T
P06	TCG CCC CAT T
P07	CGT GGT TCC C
P08	GTC CCG TTA C
P09	ACG CCC TAG T
P11	CTG TGC TGT G
P12	TGG TGG ATG TA
P13	GGT GAT GTC C
P14	TGC CTT CCA T
P15	GGG TTG CCG T
P21	CCC TGT CTC T
P27	ACC TCG AGC A
P28	TCG TAG CCA A
P33	GTA AAA CGA CGG CCA GT
P35	TGC GCA ACG TTG TTG
P36	ATA CCA AAC GAC GAG
P98	GAA CGA CGC A
P105	CAG TCG CGT G
P117	GGG ATC GTG T
P136	AGC CTC GAT T
P137	ATC TGC GAC A
P145	TAG CGG CTA C
P146	TGA CAT GCG A
P153	AAG CGA TGT T
P161	CGG ATG CCT T
P163	ACG CCT ACG T
P166	GTG ACG GAC T
P210	AAA TGC GGC A
P217	CGC TCA TTC G
P220	ACA GTG CTG G
P229	CAG CAT CCC A
P231	ATG ACG CTC C
P232	CCG CTT GTT G
P242	ATG ATA CGC C
P245	CTA GGT CTC C
P252	TCA GGC TTC C
P255	ACG TTT TCC C
P265	ACG ACA GGC T
P300	ATT GCA GCC A
P312	GTG CGC CTA A
P313	AAA GCC GTC C
P314	GGT GCT CTG A
P319	ACT ACA CGA C
P321	GTT TCG CAC C
P322	CTC CAG ACA G
P323	GTG CAC CCT T
P324	CAC GCC AAA G
P326	TAC GTG CTG G
P348	CGA AAA GCG G
P353	ATA CGG GCA A
P363	AGG TGG TTC C
P371	GCC GCT TAG T
P372	AAC ACG GTC A
P379	AAC TCC GGG A
P389	GTC AGG GTG A
P391	AGA TGG GAG G
P394	CGA CTC CAA C
P395	CTG GTT CTG T
P402	GCG TTG TCC A
P414	AAG TGA GGC A
P437	CGG ATC GAC A
P441	ACC GAG TCG A
P482	AGT CGG GCA A
S05	CCG GCT CTT G
S11	CAG TGG AAT G
S12	GCG ACG CCT A
S16	CGT TGG ATG C
S19	TAC GGC TGG C
S21	CCC TAC CGA C
S23	CCA CGC TAT A
S29	CCA GAC AAG C
S32	CCC ACG GAT C
S34	GAT AGC CGA C
S35	AGT CGC TCA T
S39	TCG GCC TGC T
S41	GCC GAA AAG C
S42	CCC AGA ACA C
S44	ATT CGG CCG C
S45	CAC GTC GGA G
S60	CCG CCT CCT T
S62	GAT CCG CGT G
S64	AAC TGG CGA C
S66	GCT CAC CCT A
S69	CAT CGA ACC G

Combination Code	Primer Combination
C03	B305 + B389
C04	P252 + B389
C06	P265 + B389
C07	B388 + S60
C08	B388 + B389
C09	OP07 + OP09
C10	OP03 + OP12
C13	P312 + P348
C14	B302 + K05
C15	B302 + K08
C16	B389 + K05
C19	P217 + K08

Primers Used for RAPD Analysis of 'Cayuga White' x 'Aurore'

Primers Used in Combination

Primers Used for RAPD Analysis of
'Cayuga White' x 'Aurore'