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My research program addresses pathogenesis of economically important
fungal pathogens of grapevine. The main pathosystems we focus
on involve Botrytis cinerea and Erysiphe
necator (powdery mildew),
the necrotrophic rotter and the stealthy biotroph, respectively. Our
goal is to use genetic, genomic, proteomic, and epidemiological
approaches to decipher the molecular requirements for compatibility. We
are aided in this pursuit by temporal incompatibility in the fruit
for both pathogens: Botrytis infects early but waits quiescently
while the immature fruit develops until a certain point late in
fruit development when it is finally able to flex its pathogenic
muscles and destroy the fruit; Erysiphe infects early, causes symptoms
early, and then at a very specific time in fruit development is
neither able to colonize the host any further nor initiate new
infections.
We are also developing publicly available tools for studying both
the pathogen and the host. These include systems for global
expression analysis, molecular markers, and random mutagenesis libraries. In
addition, we aim to utilize the natural variation contained in the
PGRU Vitis germplasm collection to identify novel sources of resistance
to a wide array of fungal pathogens.
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