Phyllosticta cirri.avi
Spores of Phyllosticta ampelicida emanate from pycnia in cirri when the surface is flooded with water.

Behavior of Phyllosticta ampelicida spores in contact with substrata of low and high wettabilities as observed using an inverted light microscope.  Microscope focus was maintained on the inner chamber surface as spores drifted downward.

Phyllosticta hydrophobic.avi
Spores in water attach immediately (≤ 0.03 sec) upon contact with a poorly wettable (hydrophobic) octadecylsilane treated glass surface.  Seven spores are already attached and immobile; 'out-of-focus' spores drift into view and immediate contact and attach the substratum.  These spores will ultimately germinate. Time is hr:min: sec.

Phyllosticta hydrophilic.avi
Spores in water do not attach to highly wettable (hydrophilic) glass surface.  These spores move about the chamber surface in a Brownian-like motion and seldom attach or germinate.  Time is hr:min: sec.

Phyllosticta germination.avi
Spores of Phyllosticta ampelicida on hydrophobic surfaces germinate, forming short germ tubes that swell to form appressoria.  Maturing appressoria are melanized.  Cytoplasm in the spores migrates into the developing appressoria that are eventually separated from the spores by a septum.