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Siraprapa Mahanil
Postdoctoral Associate
Cornell University
Department of Plant Pathology
New York State Agriculture Experiment Station
Geneva, NY 14456
2007. MS/PhD, Plant Breeding, School of Crop Production Technology, Suranaree University of Technology, Thailand
1999. BSc, Crop Production Technology, School of Crop Production Technology, Suranaree University of Technology, Thailand
E-mail:
sm379@cornell.edu
Host: Lance Cadle-Davidson
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| Research
Focus |
I am interested in the interactions between pathogens and disease resistance gene(s) at the molecular level. In my earlier research, I studied the mapping of downy mildew resistance genes with particular emphasis on resistance gene analogs (RGAs) in North American grapevine species such as Vitis cinerea and V. rupestris. I presently am using various molecular technologies to identify novel resistance genes or disease resistant cultivars. The QTL mapping of SSRs, AFLP and SNP markers will provide markers associated with disease resistance genes. Thus, marker assisted selection (MAS) will allow us to screen non-transgenic breeding populations rapidly and eliminate susceptible individuals, increasing the efficiency of combining disease resistance with horticultural and quality traits. Also, gene silencing techniques will be use to identified function of powdery mildew resistance genes (such as Mlo, Pmr) in grapevine. In parallel, I am studying on gene-for-gene resistance to grape powdery mildew. We will characterize the plant-pathogen interactions, in term of race-specificity and microscopic analysis for each of three resistance source from Asian and North American Vitis spp.
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| Professional
Experience |
| 2007-present. |
Postdoctoral Associate, Department of Plant Pathology, Cornell University. Supervisor: Lance Cadle-Davidson. I am studying in the development of powdery mildew resistant grapevines through marker assisted selection. |
| 2007. |
Guest lecturer and laboratory instructor, Taught Plant Breeding Laboratory, Suranaree University of Technology, Thailand. |
| 2004-2006. |
Graduate research assistant, Department of Horticultural Sciences, Cornell University, Supervisor: Bruce I. Reisch and Christopher L. Owens. I studied genotype identification by using molecular markers development of genetic linkage map in Vitis spp. |
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| 2003. |
Research Scholar fellow, Department of Horticultural Sciences, Cornell University, Supervisor: Bruce I. Reisch. I studied inter-specific hybridization in grapevine and disease resistance breeding program. |
| 2003-2007. |
Research Scholar in Royal Golden Jubilee Ph.D. Program, Thailand Research Fund, Advisor: Paisan Laosuwan, Piyada Thipyapong, Bruce I. Reisch and Christopher L. Owens. I studied cloning of candidate resistance gene analogs (RGAs) in grapevine, developing genetic linkage map in V. spp. by using molecular markers, and inheritance of downy mildew resistance in the grapevine (V. spp.). |
| 2001-2003. |
Research Scholar from the Center for Agricultural Biotechnology, Thailand, Advisor: Piyada Thipyapong. I studied transgenic tomato modified for overexpression of tomato polyphenol oxidase to increase resistance to common cutworm. |
| 2000-2002. |
Teaching assistant, Taught Physiology of Crop Production, Plant Pests Technology I, Plant Growth Regulator, Economic Ornamental Crop Production, Plant Tissue Culture, Plant Breeding Laboratory, Physiology of Environmental Stress, Genetic Engineering for Crop Improvement and Advanced Methods in Plant Breeding Laboratory, Suranaree University of Technology, Thailand. |
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| Professional
Activities |
Professional Assignments, Honors, and Awards
| 2007. |
Certification for outstanding research (oral) presentation at the RGJ-Ph. D. Congress VII, Pattaya, Thailand. |
| 2007. |
Certification for excellence in academics and research of Institute of Agricultural Technology, Suranaree University of Technology, Thailand. |
Professional Societies and Working Groups
Research Scholar from Royal Golden Jubilee Ph.D. Program, Thailand Research Fund.
Research Scholar from the Center for Agricultural Biotechnology, Thailand.
American Phytopathological Society
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| Publications |
Mahanil, S., Reisch, I. B., Owens, L. C., Thipyapong, P., and Laosuwan, P. 2007. Resistance Gene Analogs (RGAs) from Vitis cinerea, V. rupestris and V. hybrid ‘Horizon’. Am. J. Enol. Vitic. 58:4:484-493.
Mahanil, S., Thipyapong, P., Reisch, B., Owens, C. and Laosuwan, P. 2007. Cloning of resistance gene analogs (RGAs) in Vitis spp. RGJ-Ph. D. Congress VIII. Pattaya, Thailand.
Mahanil, S., Thipyapong, P., Reisch, B., Owens, C. and Laosuwan, P. 2006. NBS-LRR type Resistance Gene Analogs (RGAs) from Vitis cinerea, V. rupestris and ‘Horizon’. The international workshop of tropical and subtropical fruits. Chaing Mai, Thailand.
Mahanil, S., Thipyapong, P., Reisch, B., Owens, C. and Laosuwan, P. 2006. Cloning of Resistance Gene Analogs (RGAs) in Grapevine Vitis rupestris and ‘Horizon’. Plant & Animal genome XIV conference. California, USA.
Mahanil, S., Thipyapong, P., Reisch, B., Owens, C. and Laosuwan, P. 2005. Cloning of Resistance Gene Analogs (RGAs) in Grapevine Vitis cinerea. 1st International Grape Genomics symposium. Missouri, USA.
Thipyapong, P., Mahanil, S., Bhonwong, A., Attajarusit, J. and Steffens, J.C. 2004. Increasing resistance of tomato to Lepidopteran insects by overexpression of Polyphenol Oxidase. 6th World congress on the processing tomato & 9th ISHS symposium on the processing tomato. Melbourne, Australia.
Mahanil, S. and Thipyapong, P. 2004. Cloning of candidate resistance gene analogs (RGAs) for downy mildew resistance in grape (Vitis spp.). The 4th National Symposium on Graduate Research. Chaing Mai, Thailand.
Mahanil, S. and Thipyapong, P. 2004. Cloning of candidate resistance gene analogs (RGAs) for downy mildew resistance in grape (Vitis spp.). The 4th National Symposium on Graduate Research. Chaing Mai, Thailand.
Thipyapong, P., Mahanil, S., Attajarusit, J. and Steffens, J.C. 2003. Overexpression of Polyphenol Oxidase in Transgenic Tomato Plants Increase Resistance to Common Cutworm (Spodoptera litura (F.)). Plant biology 2003. Hawaii, USA.
Thipyapong, P., Mahanil, S., Attajarusit, J. and Steffens, J.C. 2003. Overexpression of Polyphenol Oxidase in Transgenic Tomato Plants Increase Resistance to Common Cutworm (Spodoptera litura (F.)). Plant biology 2003. Hawaii, USA.
Mahanil, S. 2002. Response of Tomato Polyphenol Oxidases to Common Cutworm (Spodoptera litura (F.)) Infestation. The 3rd National Symposium on Graduate Research. Nakhon Ratchasima, Thailand.
Mahanil, S., Attajarusit, J., Stout, M.J. and Thipyapong, P. (submitted). Overexpression of Tomato Polyphenol Oxidase Increases Resistance to Common Cutworm.
Mahanil, S., Thipyapong, P. and Laosuwan, P. (submitted). General and specific combining ability from North Carolina design II of downy mildew (Plasmopara viticola) resistance in table grape (Vitis spp.).
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